7LFA

Fab 3B6 bound to ApoL1 NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-11-30
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	52.753, 71.867, 123.436
Unit cell angles	80.16, 85.05, 90.16

Refinement procedure

Resolution	70.799 - 1.857
R-factor	0.2065
Rwork	0.205
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vl5
RMSD bond length	0.011
RMSD bond angle	1.310
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.990	2.044
High resolution limit [Å]	1.857	1.858
Rmerge	0.059	0.574
Rmeas		0.701
Rpim	0.059	0.496
Number of reflections	82724	4136
<I/σ(I)>	3.6	1.6
Completeness [%]	89.6	52.2
Redundancy	3.2	3
CC(1/2)	0.998	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	40% MPD, 5% PEG 8000, 0.1M Na Cacodylate pH 6.5