7LDQ
Crystal structure of putative NAD(P)H-flavin oxidoreductase from Haemophilus influenzae R2846
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-06 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.100, 84.838, 53.977 |
| Unit cell angles | 90.00, 115.83, 90.00 |
Refinement procedure
| Resolution | 31.950 - 1.150 |
| R-factor | 0.1432 |
| Rwork | 0.142 |
| R-free | 0.15880 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.801 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.101 | |
| Number of reflections | 74481 | 3643 |
| <I/σ(I)> | 18.8 | 3.2 |
| Completeness [%] | 98.0 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.811 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 40% PEG300 |
