7LD6
G150A Pseudomonas fluorescens isocyanide hydratase (G150A-1) at 274K, Phenix-refined
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 274 |
| Detector technology | PIXEL |
| Collection date | 2018-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.775 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.249, 58.133, 69.087 |
| Unit cell angles | 90.00, 112.78, 90.00 |
Refinement procedure
| Resolution | 34.590 - 1.300 |
| R-factor | 0.1205 |
| Rwork | 0.120 |
| R-free | 0.14340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ni5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.799 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.110 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.057 | 1.259 |
| Number of reflections | 100703 | 4869 |
| <I/σ(I)> | 10.4 | 1.2 |
| Completeness [%] | 98.0 | 95.8 |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.998 | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 25% PEG 3350, 200 MM MAGNESIUM CHLORIDE, 100MM TRIS-HCL, PH 8.6, 2 MM Dithiothreitol |
