7LCZ
Crystal structure of the ARM domain from Drosophila SARM1 in complex with NMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.921, 50.788, 76.054 |
| Unit cell angles | 103.52, 101.90, 95.26 |
Refinement procedure
| Resolution | 37.670 - 1.650 |
| R-factor | 0.2125 |
| Rwork | 0.212 |
| R-free | 0.22950 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.473 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.880 | 1.600 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 83026 | 3201 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 83.5 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M SPG buffer (pH 8.0) and 25% PEG1500 |
