7LCM

Receiver Domain of RssB bound to beryllofluoride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	46.329, 46.329, 95.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.120 - 1.910
R-factor	0.2065
Rwork	0.202
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7l9c
RMSD bond length	0.008
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.120	1.950
High resolution limit [Å]	1.900	1.910
Rpim	0.028	0.366
Number of reflections	9736	908
<I/σ(I)>	21	1.7
Completeness [%]	99.5
Redundancy	9.3
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	298	0.2M lithium sulfate, 22% PEG 3,350, and 0.1M Tris-HCl pH 8.8