7LCD
Crystal structure of human polymerase eta complexed with syn N7-acetophenoneguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.785, 98.785, 81.876 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.780 - 1.980 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o3n |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.936 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rpim | 0.057 | 0.427 |
| Number of reflections | 31506 | 1580 |
| <I/σ(I)> | 24.2 | 2.48 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | |
| CC(1/2) | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES pH 6.5 20% PEG2000 MME 5 mM magnesium chloride |
