7LC5
Crystal structure of epoxyqueuosine reductase QueH from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.224, 105.181, 73.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.490 - 1.500 |
| R-factor | 0.1963 |
| Rwork | 0.196 |
| R-free | 0.21130 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.805 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 1.551 |
| High resolution limit [Å] | 1.497 | 1.497 |
| Rmerge | 0.044 | 0.708 |
| Rmeas | 0.046 | 0.739 |
| Rpim | 0.013 | 0.207 |
| Number of reflections | 33575 | 3276 |
| <I/σ(I)> | 36.83 | 4.85 |
| Completeness [%] | 99.3 | |
| Redundancy | 13 | |
| CC(1/2) | 1.000 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 mM Bis-Tris, pH 5.5, 200 mM lithium sulfate monohydrate, 25% PEG3350 |
