7LBJ

Crystal structure of octaprenyl diphosphate synthase from Stenotrophomonas maltophilia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-12
Detector	RAYONIX MX-300
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	52.060, 60.330, 164.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.670 - 1.850
R-factor	0.153
Rwork	0.151
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ts7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MoRDa
Refinement software	PHENIX (1.19rc4-4035)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.670	29.670	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.049	0.032	0.260
Rmeas	0.054	0.036	0.331
Total number of observations	225899
Number of reflections	44553	571	2898
<I/σ(I)>	21.64	40.62	2.84
Completeness [%]	98.8	95.8	88.5
Redundancy	5.07	4.982	2.109
CC(1/2)	0.999	0.999	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	StmaA.01095.a.B1.PW38725 at 18.4 mg/ml was mixed 1:1 with MCSG-1 D3 (313352d3): 0.2 M NaCl, 0.1 M Bis-Tris HCl pH 6.5, 25 (w/v) PEG 3350. Cryo: 15% EG: puck/pin id: yls9-8, aps21idg, 20191212.