7LAO
Crystal structure of aminoglycoside acetyltransferase AAC(3)-IIb
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.174, 61.434, 112.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.320 - 1.920 |
R-factor | 0.1823 |
Rwork | 0.180 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bc3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.260 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.080 | 0.332 |
Rpim | 0.037 | 0.159 |
Number of reflections | 22563 | 935 |
<I/σ(I)> | 26.2 | 3.13 |
Completeness [%] | 95.8 | 79.8 |
Redundancy | 5.4 | |
CC(1/2) | 0.998 | 0.983 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M citric acid, Bis-tris propane pH 6.4, 25% (w/v) PEG3350, 2 mM magnesium chloride, 1 mM tobramycin |