7LAM
Crystal structure of Campylobacter jejuni Cj0843c lytic transglycosylase in complex with N,N',N''-triacetylchitotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97935 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 178.242, 178.242, 178.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.720 - 2.310 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cf9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.720 | 29.710 | 2.370 |
| High resolution limit [Å] | 2.310 | 10.310 | 2.310 |
| Rmerge | 0.149 | 0.046 | 2.346 |
| Rmeas | 0.156 | 0.048 | 2.454 |
| Rpim | 0.043 | 0.013 | 0.708 |
| Total number of observations | 6043 | 32913 | |
| Number of reflections | 41293 | 490 | 2871 |
| <I/σ(I)> | 13 | 46.3 | 0.9 |
| Completeness [%] | 99.5 | 96 | 94.3 |
| Redundancy | 13.4 | 12.3 | 11.5 |
| CC(1/2) | 0.998 | 0.999 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 sodium citrate buffer pH 5.1-6.0, and 25-33% PEG 600. Protein was 10mg/ml in 10 mM HEPES pH 8.0 and 200 mM ammonium sulfate |
