7LA6

THE STRUCTURE OF A SENSOR DOMAIN OF A HISTIDINE KINASE (VxrA) FROM VIBRIO CHOLERAE O1 BIOVAR ELTOR STR. N16961, N239 deletion mutant

Replaces:  7KB4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	87.654, 87.654, 106.223
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.420 - 1.980
R-factor	0.1827
Rwork	0.181
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r7q
RMSD bond length	0.017
RMSD bond angle	1.524
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000	2.030
High resolution limit [Å]	1.980	1.980
Rmerge	0.071	0.787
Rmeas	0.076	0.845
Rpim	0.027	0.302
Number of reflections	29036	1416
<I/σ(I)>	42.2	2.4
Completeness [%]	99.9	99.9
Redundancy	8	7.8
CC(1/2)	1.000	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2 M LITHIUM SULFIDE, 0.1 M SODIUM, CACODYLATE:HCL, 30% (W/V) PEG 400