7LA0
Pseudomonas fluorescens G150A isocyanide hydratase (G150A-2) at 274K, Refmac5-refined
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 274 |
| Detector technology | PIXEL |
| Collection date | 2018-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.775 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.155, 57.870, 68.988 |
| Unit cell angles | 90.00, 112.74, 90.00 |
Refinement procedure
| Resolution | 38.999 - 1.250 |
| Rwork | 0.124 |
| R-free | 0.15240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ni5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.473 |
| Data reduction software | autoXDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmeas | 0.071 | 1.851 |
| Number of reflections | 109380 | 5115 |
| <I/σ(I)> | 7 | 0.8 |
| Completeness [%] | 95.6 | 90.1 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.998 | 0.317 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 23% PEG 3350, 100MM TRIS-HCL PH 8.6, 200 MM MAGNESIUM CHLORIDE AND 2 MM DITHIOTHREITOL |
