7L8L

Crystal structure of human R152H GPX4-U46C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	32.698, 70.100, 35.718
Unit cell angles	90.00, 116.26, 90.00

Refinement procedure

Resolution	35.050 - 1.610
R-factor	0.1839
Rwork	0.180
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7l8k
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.100	1.650
High resolution limit [Å]	1.610	1.610
Number of reflections	17072	1158
<I/σ(I)>	10.5
Completeness [%]	92.1
Redundancy	3.3
CC(1/2)	0.970	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.9	293	0.2 M sodium thiocyanate, pH 6.9, and 20% (w/v) PEG 8000