7L82
x-ray structure of the psychrobacter cryohalolentis Pcryo_0637 N-acetyltransferase in the presene of its reaction tetrahedral intermediate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-03 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 97.237, 97.237, 66.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.699 - 1.400 |
| Rwork | 0.155 |
| R-free | 0.18050 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7l7y |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.069 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.399 | 1.400 |
| Number of reflections | 45470 | 8481 |
| <I/σ(I)> | 20.2 | 2.4 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 5.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23-26% MPD, 100 mM HEPES crystals incubated with 5 mM acetyl-coenzyme A and 5 mM UDP-2-acetamido-4-amino-2,4,3-trideoxy-D-glucose |
