7L7Z
x-ray structure of the N-acetyltransferase Pcryo_0637 from psychrobacter cryohalolentis in the presence of coenzyme A and UDP-di-N-acetyl-bacillosamine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-19 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54718 |
| Spacegroup name | H 3 |
| Unit cell lengths | 96.898, 96.898, 66.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.983 - 1.550 |
| Rwork | 0.162 |
| R-free | 0.19030 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 7l7y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.634 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 33312 | 5566 |
| <I/σ(I)> | 19.6 | 3 |
| Completeness [%] | 98.1 | 95 |
| Redundancy | 5.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 23-26% MPD, 100 mM HEPES. protein incubated with 5 mM coenzyme A and 20 mM UDP-2,4-diacetamido-2,4,6-trideoxy-D-glucose |
