7L67

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Fuc-(alpha1-3)-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.120, 54.245, 35.805
Unit cell angles	90.00, 94.99, 90.00

Refinement procedure

Resolution	27.120 - 1.200
R-factor	0.15
Rwork	0.148
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jue
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.120	27.120	1.220
High resolution limit [Å]	1.200	6.570	1.200
Rmerge	0.056	0.067	0.151
Rmeas	0.060	0.075	0.165
Rpim	0.023	0.032	0.065
Total number of observations	274139	1621	12395
Number of reflections	40273	260	1948
<I/σ(I)>	20.2	29.9	9
Completeness [%]	99.0	96.9	97.7
Redundancy	6.8	6.2	6.4
CC(1/2)	0.997	0.970	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 15mM Fuc-(alpha1-3)-GlcNAc. Reservoir solution: 12.5% Peg 8K, 0.1M MES, pH 6.0