7L66

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Methyl-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	54.484, 66.633, 34.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.440 - 1.750
R-factor	0.1902
Rwork	0.189
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.440	34.440	1.780
High resolution limit [Å]	1.750	9.090	1.750
Rmerge	0.072	0.053	0.384
Rmeas	0.075	0.056	0.399
Rpim	0.021	0.017	0.108
Total number of observations	173468	1313	8923
Number of reflections	13185	125	670
<I/σ(I)>	20.2	30.7	6.9
Completeness [%]	99.7	99.1	98.2
Redundancy	13.2	10.5	13.3
CC(1/2)	0.998	0.989	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 3mg/ml protein in 5 mM CaCl2, 10 mM Tris, pH 8.0, 25 mM NaCl, and 50mM alpha-Me-GlcNAc. Reservoir solution: 20% Peg 8K, 0.1M MES, pH 6.0