7L65

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Methyl-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	32.810, 56.326, 34.330
Unit cell angles	90.00, 112.08, 90.00

Refinement procedure

Resolution	30.400 - 1.350
R-factor	0.1371
Rwork	0.135
R-free	0.16980
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.400	30.400	1.370
High resolution limit [Å]	1.350	7.390	1.350
Rmerge	0.064	0.083	0.116
Rmeas	0.069	0.094	0.126
Rpim	0.026	0.041	0.049
Total number of observations	174816	977	8232
Number of reflections	25247	166	1247
<I/σ(I)>	18.8	23.7	9.7
Completeness [%]	99.0	96.7	97.4
Redundancy	6.9	5.9	6.6
CC(1/2)	0.997	0.974	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5 mM CaCl2, 10 mM Tris, pH 8.0, 25 mM NaCl, and 50mM alpha-MeGlcNAc. Reservoir solution: 15% Peg 8K, 0.1M MES, pH 6.0