7L63

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with L-fucose-(alpha 1-2)-D-galactose-(beta1-4)-D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.514, 54.479, 37.172
Unit cell angles	90.00, 101.31, 90.00

Refinement procedure

Resolution	36.450 - 1.650
R-factor	0.1777
Rwork	0.176
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.450	36.450	1.680
High resolution limit [Å]	1.650	9.040	1.650
Rmerge	0.056	0.039	0.303
Rmeas	0.060	0.042	0.328
Rpim	0.023	0.016	0.124
Total number of observations	113470	736	5492
Number of reflections	16058	108	787
<I/σ(I)>	17.9	35.1	5.1
Completeness [%]	98.4	97.9	99.4
Redundancy	7.1	6.8	7
CC(1/2)	0.999	0.999	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 15mM L-fucose-(alpha 1-2)-D-galactose-(beta1-4)-D-glucose. Reservoir solution: 12.5% Peg 8K, 0.1M MES, pH 6.0