7L62

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with L-fucose-(alpha 1-2)-D-galactose-(beta1-4)-D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.084, 54.065, 35.876
Unit cell angles	90.00, 95.00, 90.00

Refinement procedure

Resolution	33.950 - 1.550
R-factor	0.1372
Rwork	0.136
R-free	0.16680
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7jue
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.740	35.740	1.570
High resolution limit [Å]	1.550	8.480	1.550
Rmerge	0.059	0.058	0.128
Rmeas	0.064	0.063	0.139
Rpim	0.024	0.025	0.053
Total number of observations	129934	807	5673
Number of reflections	18748	125	859
<I/σ(I)>	17.5	28.7	6.6
Completeness [%]	98.9	96.9	94.3
Redundancy	6.9	6.5	6.6
CC(1/2)	0.998	0.992	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 15mM L-fucose-(alpha 1-2)-D-galactose-(beta1-4)-D-glucose. Reservoir solution:12.5% Peg 8K, 0.1M MES, pH 6.0