7L5S

Crystal Structure of Haemophilus influenzae MtsZ at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953722
Spacegroup name	P 21 21 21
Unit cell lengths	60.470, 100.156, 132.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.860 - 2.089
R-factor	0.152
Rwork	0.151
R-free	0.18220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dms
RMSD bond length	0.003
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (dev_3126)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.230	48.230	2.150
High resolution limit [Å]	2.089	9.100	2.090
Rmerge	0.137	0.086	0.629
Rmeas	0.143	0.090	0.657
Rpim	0.039	0.026	0.187
Total number of observations		8292	38818
Number of reflections	48375	690	3372
<I/σ(I)>	13	23.8	4.1
Completeness [%]	99.3	99.5	91
Redundancy	13.3	12	11.5
CC(1/2)	0.997	0.993	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Na-citrate pH 5.5, 100 mM methionine sulfoxide, 22.5 % PEG3350