7L5L
Crystal structure of the DiB-RM protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 43 |
| Unit cell lengths | 44.970, 44.970, 158.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.120 - 2.010 |
| R-factor | 0.1933 |
| Rwork | 0.191 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.516 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.120 | 39.130 | 2.040 |
| High resolution limit [Å] | 2.010 | 5.450 | 2.010 |
| Rmeas | 0.095 | 0.034 | 1.097 |
| Rpim | 0.039 | 0.014 | 0.460 |
| Total number of observations | 106493 | 5269 | 5339 |
| Number of reflections | 20728 | 1037 | 1063 |
| <I/σ(I)> | 12.9 | 35.6 | 1.6 |
| Completeness [%] | 99.3 | 97.4 | 99.7 |
| Redundancy | 5.1 | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 1.5 M lithium sulfate, 5% isopropanol, 0.1 M cesium chloride |
