7L5K
Crystal structure of the DiB-RM protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.256, 69.256, 80.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.320 - 1.860 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.22240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.638 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.320 | 33.330 | 1.890 |
| High resolution limit [Å] | 1.860 | 5.040 | 1.860 |
| Rmeas | 0.077 | 0.057 | 0.469 |
| Rpim | 0.036 | 0.028 | 0.226 |
| Total number of observations | 148895 | 7345 | 6905 |
| Number of reflections | 36055 | 1784 | 1776 |
| <I/σ(I)> | 9.1 | 15.3 | 2 |
| Completeness [%] | 99.8 | 98.8 | 98.7 |
| Redundancy | 4.1 | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2 M ammonium sulfate, 2.5% 2-propanol, 5% w/v n-Dodecyl-b-D-maltoside |
