7L5I
Crystal Structure of Haemophilus influenzae MtsZ at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953736 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.200, 93.681, 132.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.733 |
| R-factor | 0.1329 |
| Rwork | 0.132 |
| R-free | 0.16030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eu1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.840 | 46.840 | 1.770 |
| High resolution limit [Å] | 1.730 | 9.010 | 1.730 |
| Rmerge | 0.108 | 0.055 | 0.658 |
| Rmeas | 0.113 | 0.058 | 0.685 |
| Rpim | 0.031 | 0.017 | 0.188 |
| Total number of observations | 1007899 | ||
| Number of reflections | 75666 | 644 | 3691 |
| <I/σ(I)> | 17.4 | ||
| Completeness [%] | 99.3 | 99.5 | 87.9 |
| Redundancy | 13.3 | 11.3 | 12.3 |
| CC(1/2) | 0.999 | 0.998 | 0.904 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, 22 % PEG 3350 |
