7L59

Crystal structure of the dark-adapted full-length bacteriophytochrome XccBphP-G454E variant from Xanthomonas campestris in the Pfr state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	I 41
Unit cell lengths	182.035, 182.035, 40.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 2.680
R-factor	0.198
Rwork	0.196
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5akp
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.510	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.096	1.431
Rmeas	0.100	1.479
Rpim	0.025	0.374
Number of reflections	18944	2464
<I/σ(I)>	15.9	1.9
Completeness [%]	100.0	100
Redundancy	15.3	15.5
CC(1/2)	0.999	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	294	30% (w/v) pentaerythritol propoxylate (5/4 PO/OH), 0.1 M MES