7L4H
Crystal structure of the DRM2-CTG DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-07-02 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.888, 230.379, 54.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 115.190 - 2.560 |
| R-factor | 0.2119 |
| Rwork | 0.209 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4onj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.702 |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 115.190 | 115.190 | 2.680 |
| High resolution limit [Å] | 2.560 | 8.880 | 2.560 |
| Rmerge | 0.164 | 0.059 | 1.373 |
| Rmeas | 0.188 | 0.070 | 1.560 |
| Rpim | 0.089 | 0.036 | 0.723 |
| Total number of observations | 103577 | 2367 | 12051 |
| Number of reflections | 23715 | 624 | 2729 |
| <I/σ(I)> | 7.3 | 21.8 | 1.2 |
| Completeness [%] | 98.6 | 96.1 | 94.3 |
| Redundancy | 4.4 | 3.8 | 4.4 |
| CC(1/2) | 0.993 | 0.996 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2 M Potassium iodide,20% w/v Polyethylene glycol 3,350, pH 7.0. |
