7L4F

Crystal structure of the DRM2-CAT DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2018-07-28
Detector	Nonius Kappa CCD
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	172.216, 70.833, 103.735
Unit cell angles	90.00, 98.02, 90.00

Refinement procedure

Resolution	85.267 - 2.550
R-factor	0.2104
Rwork	0.208
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4onj
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	102.720	102.720	2.650
High resolution limit [Å]	2.550	9.190	2.550
Rmerge	0.084	0.038	1.476
Rmeas	0.092	0.041	1.602
Rpim	0.035	0.016	0.615
Total number of observations	272465	5840	30448
Number of reflections	40496	923	4568
<I/σ(I)>	14	43.4	1
Completeness [%]	99.7	99.5	99.9
Redundancy	6.7	6.3	6.7
CC(1/2)	0.999	0.998	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Sodium acetate trihydrate (pH 7.0) and 12% w/v Polyethylene glycol 3,350.