7L22
Structure of chloride soak form of ArrX from Chrysiogenes arsenatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.896, 54.477, 90.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.720 - 1.925 |
| Rwork | 0.169 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6x6b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.720 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 20389 | 1173 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.0 | |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium chloride, 0.1 M Tris pH 8.5, 27% (w/v) PEG 3350 |
