7L1W
Unlocking the structural features for the exo-xylobiosidase activity of an unusual GH11 member identified in a compost-derived consortium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | P 63 |
| Unit cell lengths | 96.950, 96.950, 40.899 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.990 - 1.710 |
| R-factor | 0.1609 |
| Rwork | 0.159 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vqj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.856 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (Phenix.Refine) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.990 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.116 | 1.956 |
| Rpim | 0.027 | 0.546 |
| Number of reflections | 23891 | 2358 |
| <I/σ(I)> | 19.92 | 1.52 |
| Completeness [%] | 99.9 | |
| Redundancy | 18.1 | |
| CC(1/2) | 0.999 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M MES pH 6.5 and 30% (w/v) PEG 4000 |
