7L17
Crystal structure of sugar-bound melibiose permease MelB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.364, 126.364, 104.334 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.890 - 3.050 |
| R-factor | 0.2627 |
| Rwork | 0.259 |
| R-free | 0.29350 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.536 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (dev_3936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.895 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.109 | 1.370 |
| Rmeas | 0.114 | 1.430 |
| Rpim | 0.034 | 0.419 |
| Number of reflections | 18680 | 3342 |
| <I/σ(I)> | 20.5 | 2.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 21 | 22.5 |
| CC(1/2) | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297.15 | 50 mM BaCl2, 50 mM CaCl2, 100 mM Tris-HCl, pH 8.5, and 29-32% PEG 400, 6 mM a-NPG |
