7L0Z
Spinach variant bound to DFHBI-1T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-17 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.105 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.564, 48.692, 30.612 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 75.260 - 2.100 |
| R-factor | 0.1996 |
| Rwork | 0.196 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ob3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.118 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.260 | 2.070 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 14648 | 1167 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 98.7 | 92.1 |
| Redundancy | 5.3 | |
| CC(1/2) | 0.999 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 40 mM Tris-HCl pH 7.5, 100 mM KCl, 1 mM MgCl2 |
