7L0O

Streptococcus gordonii C123 Domain(s)-Structural and Functional Analysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	100.049, 109.140, 204.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.592 - 2.700
R-factor	0.2201
Rwork	0.218
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qe5 2woy
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.592	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.154	2.102
Rmeas	0.167	2.276
Rpim	0.065	0.863
Number of reflections	60235	4493
<I/σ(I)>	8.2	1.2
Completeness [%]	97.1	99
Redundancy	6.3	6.6
CC(1/2)	0.994	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	1.5M NaCl, 0.1M citric acid, pH 4.0