7L0J

Structure of AMH bound to AMHR2-ECD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-05-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	66.376, 66.376, 125.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 2.600
R-factor	0.2426
Rwork	0.241
R-free	0.26790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1WAQ & 6MAC
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	9187	899
<I/σ(I)>	10.12
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.997	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M MES, pH 6.5, 0.2 M lithium sulfate, 17.5% PEG3350, 5% 1-butyl-3-methylimidazolium trifluoroacetate