7L04

Crystal Structure of Adeno-Associated Virus Porcine Origin capsid protein in complex with Importin-alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	79.424, 89.350, 99.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.670 - 2.260
R-factor	0.2157
Rwork	0.215
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bw0
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.670	2.330
High resolution limit [Å]	2.260	2.260
Rmerge	0.124	0.888
Number of reflections	33929	3034
<I/σ(I)>	10.1
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	0.996	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.6M Na citrate, 0.01M DTT, 0.1M HEPES pH 6.5