7KZW
Crystal structure of FTT_1639c from Francisella tularensis str. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 62 |
| Unit cell lengths | 83.412, 83.412, 36.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.610 - 1.340 |
| R-factor | 0.1775 |
| Rwork | 0.177 |
| R-free | 0.18880 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.032 | 0.488 |
| Rpim | 0.011 | 0.314 |
| Number of reflections | 32625 | 1394 |
| <I/σ(I)> | 61.4 | 1.89 |
| Completeness [%] | 98.6 | 84.5 |
| Redundancy | 8.4 | 3 |
| CC(1/2) | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | PEG 3350 25%, Citric acid 0.1M pH 3.5, 0.3M NDSB211, cryoprotectant trehalose, paratone |
