7KYX

Crystal structure of human CORO6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.548, 80.764, 96.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.240 - 1.630
R-factor	0.1745
Rwork	0.174
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b4e
RMSD bond length	0.007
RMSD bond angle	1.449
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.630	4.420	1.630
Rmerge	0.092	0.051	0.942
Rmeas	0.098	0.054	1.012
Rpim	0.032	0.018	0.360
Total number of observations	454557
Number of reflections	50011	2736	2387
<I/σ(I)>	7.7
Completeness [%]	99.5	99.7	97.4
Redundancy	9.1	9	7.4
CC(1/2)		0.998	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	30%(w/v)PEG550 MME,0.05M Magnesium Chloride, 0.1M Hepes pH7.4