7KX2

Spermidine N-acetyltransferase SpeG F149A mutant from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 2 2 2
Unit cell lengths	73.435, 135.942, 139.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.400 - 2.600
R-factor	0.253
Rwork	0.250
R-free	0.30790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jjx
RMSD bond length	0.001
RMSD bond angle	0.358
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	21877	2623
<I/σ(I)>	16.7	2.1
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.998	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2M potassium chloride pH 7.0, 20% PEG3350