7KWS
Cj1441 with NAD+ and UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.12709 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.790, 148.780, 62.340 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 46.440 - 2.090 |
| R-factor | 0.1929 |
| Rwork | 0.190 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlj |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 43332 | 2003 |
| <I/σ(I)> | 11.3 | 1.43 |
| Completeness [%] | 96.6 | 98 |
| Redundancy | 12.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294.15 | 5.0 mM UDP-glucose, 5.0 mM NAD+, 1.0 mM DTT, 32% pentaerythritol ethoxylate, 50 mM ammonium sulfate, 50 mM BIS-TRIS, 200 mM NaCl |
