7KWQ

Spermidine N-acetyltransferase SpeG R149-K152 chimera from Vibrio cholerae and hSSAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 2 2 2
Unit cell lengths	74.891, 137.903, 140.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 2.300
R-factor	0.2165
Rwork	0.215
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jjx
RMSD bond length	0.002
RMSD bond angle	0.407
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.180	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	32692	3160
<I/σ(I)>	16.4	2.4
Completeness [%]	100.0
Redundancy	4.6
CC(1/2)	0.998	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Deionized water