7KWJ

Spermidine N-acetyltransferase SpeG K23-Q34 chimera from Vibrio cholerae and hSSAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 2 2 2
Unit cell lengths	73.896, 134.965, 138.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.580
R-factor	0.2373
Rwork	0.235
R-free	0.27840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jjx
RMSD bond length	0.001
RMSD bond angle	0.370
Data reduction software	MOSFLM
Data scaling software	Aimless (1.18.2_3874)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.970	2.676
High resolution limit [Å]	2.580	2.580
Rmerge	0.056
Number of reflections	1878	202
<I/σ(I)>	13.6
Completeness [%]	98.3
Redundancy	4
CC(1/2)	0.998	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	2M sodium formate, 0.1M sodium acetate pH5.0