7KWD
Crystal structure of Thermus thermophilus alkaline phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-09-20 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 163.175, 163.175, 163.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.260 - 2.100 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e2d |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.260 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 182535 | 28026 |
| <I/σ(I)> | 11.98 | |
| Completeness [%] | 97.8 | 98.9 |
| Redundancy | 8.1 | 6.4 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M HEPES sodium pH 7.5, 0.8 M Potassium sodium tartrate tetrahydrate |
