7KV6

Surface glycan-binding protein B from Bacteroides fluxus in complex with mixed-linkage glucotriose

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	59.980, 77.222, 148.379
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.189 - 1.600
Rwork	0.178
R-free	0.20430
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.729
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.190	1.640
High resolution limit [Å]	1.600	1.610
Number of reflections	43047	2197
<I/σ(I)>	34.4
Completeness [%]	95.5
Redundancy	11.8
CC(1/2)	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	0.1 M MES pH6.6, 0.2 M ammonium sulfate, 20 % (w/v) PEG5000MME, 0.1M guanidine hydrochloride