7KV4
Surface glycan-binding protein A from Bacteroides fluxus
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.855, 116.505, 139.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.431 - 1.840 |
| Rwork | 0.204 |
| R-free | 0.23970 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.431 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 45565 | 2369 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.987 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M tris pH8.1, 0.2 M magnesium chloride, 23 % (w/v) PEG8000 |
