7KV1
Surface glycan-binding protein A from Bacteroides uniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.270, 53.583, 126.850 |
| Unit cell angles | 90.00, 93.04, 90.00 |
Refinement procedure
| Resolution | 41.810 - 1.860 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.20870 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.810 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 77609 | 3967 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.996 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.1 M bis-tris pH 5.3, 0.2 M ammonium acetate, 22 % (w/v) PEG3350, 0.01 M hexamine cobalt (III) chloride |
