7KUV

Crystal Structure of Danio rerio Histone Deacetylase 10 in Complex with Acetate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	80.480, 80.480, 245.463
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.700 - 2.150
R-factor	0.1863
Rwork	0.184
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5td7
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.700	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.138	1.326
Rpim	0.048	0.469
Number of reflections	51222	5030
<I/σ(I)>	11	1.9
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	0.997	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	10 mg/mL HDAC10, 10 mM sodium acetate, 1:1000 trypsin:HDAC10, 0.100 M potassium phosphate monobasic, 0.100 M potassium phosphate dibasic, 20% PEG3350 (w/v), HDAC10 microseed