7KUN
2'-F modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2020-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977408 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 48.783, 48.783, 82.434 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.580 |
Rwork | 0.194 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uee |
RMSD bond length | 0.030 |
RMSD bond angle | 3.483 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.038 | 0.330 |
Rmeas | 0.039 | 0.340 |
Rpim | 0.009 | 0.081 |
Number of reflections | 16165 | 770 |
<I/σ(I)> | 81.3 | 10.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.5 | 17.2 |
CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate, 50 mM Magnesium chloride |