7KUK
High resolution RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2020-07-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977408 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 48.391, 48.391, 82.304 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.670 |
Rwork | 0.202 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uee |
RMSD bond length | 0.027 |
RMSD bond angle | 3.072 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.038 | 0.328 |
Rmeas | 0.039 | 0.339 |
Rpim | 0.009 | 0.083 |
Number of reflections | 13505 | 641 |
<I/σ(I)> | 91.25 | 7 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 18.5 | 14.8 |
CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium formate, 50 mM Magnesium chloride |