7KTN

DNA Polymerase Mu, 8-oxodGTP:At Product State Ternary Complex, 10 mM Mg2+ (2160min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.229, 68.293, 111.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.086 - 1.330
R-factor	0.1425
Rwork	0.141
R-free	0.16260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.330	3.610	1.330
Rmerge	0.103	0.101	0.497
Rmeas	0.114	0.112	0.570
Rpim	0.048	0.048	0.270
Total number of observations	583554
Number of reflections	104884	5598	4610
<I/σ(I)>	14
Completeness [%]	98.9	98.9	88.4
Redundancy	5.6	5.3	3.9
CC(1/2)		0.974	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000