7KTI

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 20 uM Mn2+ (120min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.965, 68.716, 110.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.986 - 1.570
R-factor	0.1626
Rwork	0.162
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.600
High resolution limit [Å]	1.570	4.260	1.570
Rmerge	0.058	0.032	0.883
Rmeas	0.064	0.036	0.964
Rpim	0.025	0.015	0.383
Total number of observations	406563
Number of reflections	64456	3471	3176
<I/σ(I)>	10.9
Completeness [%]	99.9	99.6	100
Redundancy	6.3	6.1	6.2
CC(1/2)		0.998	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000