7KTH

DNA Polymerase Mu, 8-oxodGTP:Ct Product State Ternary Complex, 10 mM Mg2+ (2160min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.089, 68.373, 111.095
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.816 - 1.476
R-factor	0.1675
Rwork	0.166
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	M04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.476	4.020	1.480
Rmerge	0.086	0.066	0.911
Rmeas	0.095	0.073	1.007
Rpim	0.039	0.031	0.421
Number of reflections	77364	4128	3827
<I/σ(I)>	12
Completeness [%]	99.7	99.1	100
Redundancy	5.7	5.4	5.5
CC(1/2)		0.992	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000